Marx’s objective relationship theory is a great and significant enlightenment for ecological civilization construction: the scientific practice view provides an ontology premise for ecological civilization construction; the humanistic dimension requires that we must adhere to the concept of comprehensive development, transform the mode of human existence and unify the man-nature relationship and interpersonal relations; the unity of opposites between human and natural world provides methodology principle for ecological civilization construction. It has important theoretical reference value for today’s construction of socialist ecological civilization.