Xi Jinping Thought on Culture is the theoretical summary of the practical experience of the Party's leading in culture construction in the new era. It has both innovation and breakthrough in cultural theoretical viewpoints and deployment requirements in the layout of cultural work. It is clear that theory can be applied, and practice can be integrated into theory. Xi Jinping Thought on Culture comes from practice and guides practice, but there are few researches on Xi Jinping Thought on Culture guiding practice at present. This study takes national cultural park as the object of practice and interpretation, uses the thinking of theory can be applied and practice can be integrated into theory, and builds the internal connection between Xi Jinping Thought on Culture and the construction of national cultural parks. From the historical dimension, it analyzes the concept construction, planning implementation and construction promotion of national cultural parks, and believes that the construction of national cultural parks has witnessed the gradual systemization of Xi Jinping Thought on Culture since the 18th National Congress of the Party, and is the result of the gradual advancement of the major strategic deployment of cultural construction in the new era led by the Party Central Committee with Comrade Xi Jinping as its core. Furthermore, the inner relation between Xi Jinping Thought on Culture and the construction of national cultural park is further clarified, and national cultural parks are considered as an important experimental field and touchstone for Xi Jinping Thought on Culture to guide cultural practice. Therefore, in order to realize the goal of building good national cultural parks proposed by the 20th National Congress of the Party, it is necessary to fully understand the essence of Xi Jinping Thought on Culture and guide the construction of national cultural park with Xi Jinping Thought on Culture. To this end, we need to adhere to the position of Chinese culture, and ensure the correct political orientation of the construction of national cultural parks in the process of inheriting the excellent traditional Chinese culture, inheriting the revolutionary culture and developing the advanced socialist culture; We need to adhere to the principle of the people first, build the people's spiritual faith, realize the people's yearning for a better life, respect the people's creativity, and preserve the people's nature of the construction of national cultural parks; We need to strengthen cultural confidence, uphold openness and inclusiveness, adhere to integrity and innovation, consolidate cultural subjectivity, take the new cultural mission of the new era as our own responsibility, and ultimately achieve the goal of building good national cultural parks.