太湖芦苇根系中溶藻菌的分离鉴定及溶藻效果
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X703

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国家自然科学基金(41571471);常州市科技计划项目(CE20155061、WS201521)


Isolation and identification of algicidal bacteria from reed roots in Baidu Port of Taihu Lake and it's lytic effect
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    摘要:

    富含氮、磷、钾等营养物质的污水排入河流、湖泊,造成蓝藻爆发,而溶藻菌可以有效降解水体中的蓝藻污染。从蓝藻污染严重的太湖百渎港岸边芦苇根系中筛选出1株溶藻菌(Bacillus sp),命名为G6,系统发育分析表明,G6菌株与芽孢杆菌同源性最高。将培养至对数期的G6菌液以菌藻比1:10的比例,在温度28℃、光强2500 lx、光暗比12 h:12 h的条件下经光照培养箱培养7 d,对铜绿囊藻液Chla去除率可达82%。此外,G6菌株在无光照条件下也具有溶藻特性,这有利于其在缺少光照的深水域中增殖并发挥溶藻作用。G6通过分泌溶藻物质杀灭铜绿囊藻,属于间接溶藻,且溶藻物质具有热稳定性,能够在较高温度下发挥溶藻特性。

    Abstract:

    Large amounts of sewage with nitrogen, phosphorus, potassium and other nutrients into the river lake will cause the algae bloom. Algae-lysing bacteria which can effectively degrade cyanobacteria in water is a potential treatment. An algicidal bacterium against toxic Microcystis aeruginosastrain G6 is isolated from reed roots in Baidu port of Taihu Lake. According to phylogenetic analysis, strain G6(Bacillus sp) is identified as Bacillus sp.The strain G6 which cultivated to the logarithmic phase is voted to water with bacteria and algae ratio of 1:10. The Chl-a of algae fluid could be reduced up to 82% when it is placed in the light incubator of 28℃ temperature, light intensity 2 500 lux, and photoperiod 12 h:12 h for 7 days. Strain G6 has the character that it can kill the algae cells without light. It is conducive to bacteria breeding and degradate algae in the deep sea which lack of light. The strain G6 could produce substance which can dissolv algae to kill microcystis aeruginosa, which is an indirect way to kill algae. The substance has thermal stability to kill the algae cells at high temperature.

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沈红池,潘瑞松,吴旭鹏,蔡庆庆,张文艺.太湖芦苇根系中溶藻菌的分离鉴定及溶藻效果[J].土木与环境工程学报(中英文),2017,39(5):123-128. Shen Hongchi, Pan Ruisong, Wu Xupeng, Cai Qingqing, Zhang Wenyi. Isolation and identification of algicidal bacteria from reed roots in Baidu Port of Taihu Lake and it's lytic effect[J]. JOURNAL OF CIVIL AND ENVIRONMENTAL ENGINEERING,2017,39(5):123-128.10.11835/j. issn.1674-4764.2017.05.017

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  • 收稿日期:2016-11-26
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  • 在线发布日期: 2017-10-11
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