针对生活污水中内分泌干扰物（EDCs）可能引起的生物生态危害问题，以类固醇类内分泌干扰物为处理对象，应用生物生态耦合技术，进行多级AO+潜流式人工湿地处理试验，考察了水力停留时间（HRT）对CODCr、氨氮（NH4+-N）、总磷（TP）、总氮（TN）等污染物去除影响，同时，采用固相萃取法和GC-MS检测各反应器进、出水中雌酮（E1）、17β-雌二醇（E2）、雌三醇（E3）、17α-乙炔基雌二醇（EE2）4种内分泌干扰物浓度变化。结果表明，当多级AO与湿地的HRT分别为9.5 h、10.3 d时，多级AO+潜流式人工湿地对CODCr、NH4+-N、TP、TN等污染物去除率可分别达78.64%、97.16%、91.84%、90.55%，出水浓度均低于《太湖地区城镇污水处理厂及重点工业行业主要水污染物排放限值》（DB 32/1072-2017）排放浓度限值。多级AO+人工湿地对内分泌干扰物E1、E2、E3和EE2的总去除率可分别达96.04%、92.45%、85.09%、88.38%；其中，多级AO系统对E1、E2、E3和EE2的去除率分别达到75.50%、72.00%、65.08%和63.35%，且一级好氧池对4种EDCs的去除贡献最大，均为27%以上；湿地系统对前3种EDCs的去除率约为20%、EE2为25%。Alpha多样性指数分析表明，好氧池物种数量和多样性多于未加EDCs前，门分类、属分类的微生物群落物种及其丰度分析表明，群落门水平、属水平相对丰度均发生变化，其中，增加的优势菌种可能对系统中EDCs的去除起到了促进作用。
Aiming at the possible biological and ecological hazards caused by endocrine disrupting chemicals (EDCs) in domestic sewage, this study took steroid endocrine disruptors as the treatment object, and applied bio-ecological coupling technology to carry out a multi-stage AO+ subsurface flow constructed wetland treatment experiment. The effects of hydraulic residence time (HRT) on the removal of CODCr, ammonia nitrogen (NH4+-N), total phosphorus (TP) and total nitrogen (TN) were investigated. At the same time solid phase extraction (SPE) and GC-MS were used to detect the concentration changes of four endocrine disruptors, including estrone (E1), 17β-estrogen (E2), estriol (E3) and 17α-ethynylestradiol (EE2), in the effluent and inlet of each reactor. The results showed that when the HRT of multi-stage AO is 9.5h and that of wetland is about 10.3d, the removal rates of CODCr, NH4+-N, TP and TN could reach 78.64%, 97.16%, 91.84% and 90.55% respectively. The concentration is lower than the limit of discharge standard of main water pollutants for municipal wastewater treatment plant and key industries of Taihu area (DB32/1072-2017). The total removal rates of endocrine disruptors E1, E2, E3 and EE2 by multi-stage AO + constructed wetlands were 96.04%, 92.45%, 85.09% and 88.38% respectively. And the removal rates of E1, E2, E3 and EE2 by multi-stage AO system were 75.50%, 72.00%, 65.08% and 63.35% respectively. The first-stage aerobic tank in multi-stage AO contributed the most to the removal of four EDCs, all of which could reach more than 27%. The removal rate of the first three EDCs by wetland system were about 20%, and EE2 was 25%. Alpha diversity index analysis showed that the number and diversity of species in aerobic pond were more than those before EDCs were added. Species and abundance analysis of microbial communities in phylum and genus classifications showed that relative abundance of phylum and genus levels changed, and the increased dominant bacteria might play a role in promoting the removal of EDCs in the system.