游离氨对活性污泥系统中硝化性能和硝化群落结构的影响
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X703.1

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National Natural Science Foundation of China (No.51668031)


Free ammonia affects the nitrification performance and nitrifying community structure in the suspended activated system
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    摘要:

    建立了4个平行的SBR处理合成废水,游离氨(FA)浓度分别为0.5、5、10、15 mg/L,命名为S0.5、S5、S10和S15,4个系统的脱氮性能在整个实验过程中均很好(平均值为98.7%),利用FA对亚硝酸氧化细菌(NOB)的抑制作用,结合过程控制,成功在S10和S15系统中实现短程硝化。在建立短程硝化途径的过程中,S10的NAR在第79天迅速达到90.3%,S15的NAR在139天迅速达到90.5%。在S10的80~250 d和S15的140~250 d中,平均NAR分别稳定在98.8%和98.2%左右。用16S rRNA基因的高通量测序技术分析样本中硝化细菌的相对丰度和结构,结果表明,AOB和NOB丰度的变化与试验结果一致。FA不仅可以显著影响AOB和NOB的相对丰度,而且还可以抑制NOB活性。此外,还发现较低的AOB含量在FA浓度为15 mg/L时具有较高的氨底物微生物利用能力。

    Abstract:

    Four parallel SBRs were established to treat synthetic wastewater with preset concentrations of free ammonia (FA) (0.5, 5, 10 and 15 mg/L), including S0.5, S5, S10 and S15. The four systems removed ammonia well throughout the experiment (average value of 98.7%). The inhibition of FA by nitrite-oxidizing bacteria (NOB) combined with process control was used to achieve a nitrite pathway in S10 and S15. During the initiation of the nitrite pathway, the accumulation rate (NAR) increased dramatically to 90.3% on day 79 in S10 and to 90.5% on day 139 in S15. For S10 on day 80~250 and S15 on day 140~250, the average NARs were steady at approximately 98.8% and 98.2%, respectively. High-throughput sequencing of the 16S rRNA gene played an ever-increasing role in analyzing the relative abundance and structure of the nitrifying bacteria in these samples. The results showed that the changes in the abundance of AOB and NOB were consistent with our experimental results. FA affected not only the relative abundance of AOB and NOB, but also the activity of NOB. Although AOB and NOB coexisted in the four systems, AOB was still the main nitrifying bacteria. We found that a lower abundance of AOB had a higher microbial utilization capacity of ammonia substrate at 15 mgFA/L.

    参考文献
    [1] ANTHONISEN A, LOEHR R, PRAKASAM T, et al. Inhibition of nitrification by ammonia and nitrous acid[J]. Journal of Water Pollution Control Federation, 1976, 48(5):835-852.
    [2] VADIVELU V M, KELLER J, YUAN Z G. Effect of free ammonia on the respiration and growth processes of an enriched nitrobacter culture[J]. Water Research, 2007, 41(4):826-834.
    [3] VADIVELU V M, KELLER J, YUAN Z G. Effect of free ammonia and free nitrous acid concentration on the anabolic and catabolic processes of an enriched nitrosomonas culture[J]. Biotechnology andBioengineering, 2006, 95(5):830-839.
    [4] KIM D J, LEE D I, KELLER J. Effect of temperature and free ammonia on nitrification and nitrite accumulation in landfill leachate and analysis of its nitrifying bacterial community by FISH[J]. Bioresource Technology, 2006, 97(3):459-468.
    [5] JIANG Y S, POH L S, LIM C P, et al. Effect of free ammonia inhibition on process recovery of partial nitritation in a membrane bioreactor[J]. Bioresource Technology Reports, 2019, 6:152-158.
    [6] QIAN W T, PENG Y Z, LI X Y, et al. The inhibitory effects of free ammonia on ammonia oxidizing bacteria and nitrite oxidizing bacteria under anaerobic condition[J]. Bioresource Technology, 2017, 243:1247-1250.
    [7] AHN J H, YU R, CHANDRAN K. Distinctive microbial ecology and biokinetics of autotrophic ammonia and nitrite oxidation in a partial nitrification bioreactor[J]. Biotechnology and Bioengineering, 2008, 100(6):1078-1087.
    [8] PAMBRUN V, PAUL E, SPÉRANDIO M. Modeling the partial nitrification in sequencing batch reactor for biomass adapted to high ammonia concentrations[J]. Biotechnology and Bioengineering, 2006, 95(1):120-131.
    [9] YE Y Y, NGO H H, GUO W S, et al. A critical review on ammonium recovery from wastewater for sustainable wastewater management[J]. Bioresource Technology, 2018, 268:749-758.
    [10] WANG Q, NI B J, LEMAIRE R, et al. Modeling of nitrous oxide production from nitritation reactors treating real anaerobic digestion liquor[J]. Scientific Reports, 2016, 62:53-36.
    [11] WALTERS E, HILLE A, HE M, et al. Simultaneous nitrification/denitrification in a biofilm airlift suspension (BAS) reactor with biodegradable carrier material[J]. Water Research, 2009, 43(18):4461-4468.
    [12] JETTEN M S M, HORN S J, VAN LOOSDRECHT M C M. Towards a more sustainable municipal wastewater treatment system[J]. Water Science and Technology, 1997, 35(9):171-180.
    [13] VLAEMINCK S E, TERADA A, SMETS B F, et al. Nitrogen removal from digested black water by one-stage partial nitritation and anammox[J]. Environmental Science & Technology, 2009, 43(13):5035-5041.
    [14] CHUNG J, SHIM H, PARK S J, et al. Optimization of free ammonia concentration for nitrite accumulation in shortcut biological nitrogen removal process[J]. Bioprocess and Biosystems Engineering, 2006, 28(4):275-282.
    [15] CHUNG J, SHIM H, LEE Y W, et al. Comparison of influence of free ammonia and dissolved oxygen on nitrite accumulation between suspended and attached cells[J]. Environmental Technology, 2005, 26(1):21-33.
    [16] WEI D, ZHANG K, NGO H H, et al. Nitrogen removal via nitrite in a partial nitrification sequencing batch biofilm reactor treating high strength ammonia wastewater and its greenhouse gas emission[J]. Bioresource Technology, 2017, 230:49-55.
    [17] CHEN J W, ZHENG P, YU Y, et al. Enrichment of high activity nitrifers to enhance partial nitrification process[J]. Bioresource Technology, 2010, 101(19):7293-7298.
    [18] NISHIMURA F, HIDAKA T, NAKAGAWA A, et al. Removal of high concentration ammonia from wastewater by a combination of partial nitrification and anammox treatment[J]. Environmental Technology, 2012, 33(13):1485-1489.
    [19] ZHANG S J, PENG Y Z, WANG S Y, et al. Organic matter and concentrated nitrogen removal by shortcut nitrification and denitrification from mature municipal landfill leachate[J]. Journal of Environmental Sciences, 2007, 19(6):647-651.
    [20] HE Q L, GAO S X, ZHANG S L, et al. Chronic responses of aerobic granules to zinc oxide nanoparticles in a sequencing batch reactor performing simultaneous nitrification, denitrification and phosphorus removal[J]. Bioresource Technology, 2017, 238:95-101.
    [21] ZHANG C, QIN Y G, XU Q X, et al. Free ammonia-based pretreatment promotes short-chain fatty acid production from waste activated sludge[J]. ACS Sustainable Chemistry & Engineering, 2018, 6(7):9120-9129.
    [22] CAO J S, YU Y X, XIE K, et al. Characterizing the free ammonia exposure to the nutrients removal in activated sludge systems[J]. RSC Advances, 2017, 7(87):55088-55097.
    [23] SUI Q W, LIU C, ZHANG J Y, et al. Response of nitrite accumulation and microbial community to free ammonia and dissolved oxygen treatment of high ammonium wastewater[J]. Applied Microbiology and Biotechnology, 2016, 100(9):4177-4187.
    [24] KUAI L P, VERSTRAETE W. Ammonium removal by the oxygen-limited autotrophic nitrification-denitrification system[J]. Applied and Environmental Microbiology, 1998, 64:4500-4506.
    [25] Anon Coliphage Detection (proposed). In Standard Methods For The Examination of Water and Wastewater[S]. APHA, Washington, 1995.
    [26] WANG Q L, DUAN H R, WEI W, et al. Achieving stable main stream nitrogen removal via the nitrite pathway by sludge treatment using free ammonia[J]. Environmental Science & Technology, 2017, 51(17):9800-9807.
    [27] VAN HULLE S W, VOLCKE E I, TERUEL J L, et al. Influence of temperature and pH on the kinetics of the Sharon nitritation process[J]. Journal of Chemical Technology & Biotechnology, 2007, 82(5):471-480.
    [28] BALMELLE B, NGUYEN K M, CAPDEVILLE B, et al. Study of factors controlling nitrite build-up in biological processes for water nitrification[J]. Water Science and Technology, 1992, 26(5/6):1017-1025.
    [29] BOCK E. Growth of nitrobacter in the presence of organic matter. Ⅱ. Chemoorganotrophic growth of nitrobacter agilis[J]. Archives of Microbiology, 1976, 108:305-312.
    [30] EHRICH S, BEHRENS D, LEBEDEVA E, et al. A new obligately chemolithoautotrophic, nitrite-oxidizing bacterium, Nitrospira Moscoviensis sp. nov. and its phylogenetic relationship[J]. Archives of Microbiology, 1995, 164(1):16-23.
    [31] DAIMS H, PURKHOLD U, BJERRUM L, et al. Nitrification in sequencing biofilm batch reactors:lessons from molecular approaches[J]. Water Science and Technology, 2001, 43(3):9-18.
    [32] SUN H W, PENG Y Z, SHI X N. Advanced treatment of landfill leachate using anaerobic-aerobic process:Organic removal by simultaneous denitritation and methanogenesis and nitrogen removal via nitrite[J]. Bioresource Technology, 2015, 177:337-345.
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姜婷婷,王光杰,杨浩,吴常峰,孙洪伟,陈桐生.游离氨对活性污泥系统中硝化性能和硝化群落结构的影响[J].土木与环境工程学报(中英文),2021,43(1):193-202. JIANG Tingting, WANG Guangjie, YANG Hao, WU Changfeng, SUN Hongwei, CHEN Tongsheng. Free ammonia affects the nitrification performance and nitrifying community structure in the suspended activated system[J]. JOURNAL OF CIVIL AND ENVIRONMENTAL ENGINEERING,2021,43(1):193-202.10.11835/j. issn.2096-6717.2020.168

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  • 收稿日期:2020-05-21
  • 在线发布日期: 2021-02-02
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