剩余污泥中蛋白质的资源化利用是目前研究的热点，污泥预处理则是实现污泥中蛋白质释放的重要途径。为了进一步提高剩余污泥中蛋白质的溶出效果，选取热碱预处理、超声碱联合预处理、溶菌酶预处理对污泥进行溶胞，以蛋白质提取浓度为主要指标进行参数优化，并利用等电点法对粗提取蛋白进行纯化回收。结果表明：溶胞效果热碱预处理（pH值13、温度140℃、时间1.5 h，2 062.98 mg/L）>超声碱联合预处理（497.76 mg/L）>溶菌酶预处理（269.95 mg/L），且在pH值为3时热碱预处理蛋白质纯化回收率可达62.42%。试验结果表明：热碱预处理在提取效果方面较另外两种方法优势明显，具有良好的利用前景。

The resource utilization of protein in excess sludge is a research hotspot at home and abroad, and sludge pretreatment is an important way to realize protein release from sludge. In order to further improve the dissolution efficiency of protein in excess sludge, this paper selected thermal-alkali pretreatment, ultrasonic-alkali pretreatment and lysozyme pretreatment as the method of disintegration in sludge, and optimized the parameters with the protein extraction concentration as the main index. The isoelectric point method was also used to purify and recover crude protein. The results showed that the cellulolytic effect:thermal-alkali pretreatment (pH 13, temperature 140℃, time 1.5 h, 2 062.98 mg/L) > ultrasonic-alkali pretreatment (497.76 mg/L) > lysozyme pretreatment (269.95 mg/L), and the protein purification recovery of thermal-alkali pretreatment at pH 3 can reach 62.42%. It can be seen from the above test results that the thermal-alkali pretreatment has obvious advantages over the other two methods in terms of extraction effect, deserving of exploitation and utilization.

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国家自然科学基金（No.51608241）