利用污水厂好氧污泥进行锰氧化细菌的激活试验，探究pH值以及初始Mn²⁺浓度对激活效果的影响，采用高通量测序技术分析激活前后微生物群落变化，利用X射线衍射（X-ray diffraction，XRD）技术对产生的生物氧化锰进行表征，研究pH值和投加量对生物氧化锰去除乙炔基雌二醇（17α-ethinylestradiol，EE2）的影响。结果表明：pH值为7、初始Mn²⁺浓度为1 mmol/L时，Mn²⁺氧化率7 d内能达到83.3%，锰氧化菌激活效果最好。XRD结果表明：生物氧化锰主要含有MnO₂、Mn₃O₄、Na₃Mn（PO₃）CO₃等3种成分。高通量测序结果表明：经过激活后，芽孢杆菌属（0.75%）、不动杆菌属（1.26%）、假单胞菌属（1.36%）、鞘氨醇杆菌属（1.81%）、黄杆菌属（2.39%）、微杆菌属（2.97%）、气单胞菌属（7.35%）的丰度显著增加，典型的锰氧化细菌菌属总丰度达到17.89%。EE2在反应pH值为4.0、生物氧化锰投加量为20 mg/L的条件下去除效果最好，48 h后EE2的去除率可达97.7%，其中，生物氧化锰对EE2的去除率可达76.9%。

Aerobic sludge from the wastewater treatment plant was sampled as inoculation bacteria, and the effects of pH and initial Mn²⁺ concentration on the activation of manganese-oxidizing bacterium were studied. High-throughput sequencing technology was used to analyze the variation of microbial communities before and after activation. On this basis, the biogenic manganese oxides produced by manganese-oxidizing bacterium were characterized by X-ray diffraction. Besides, the effects of pH and biogenic manganese oxides dosage on the removal of EE2 by biogenic manganese oxides were analyzed. The results show that the best activation effect of manganese oxidizing bacterium is obtained when the initial Mn²⁺ concentration is 1 mmol/L at pH 7.0 and 83.3% Mn²⁺ is transformed into biogenic manganese oxides after 7 days under this condition. XRD results show that the biogenic manganese oxides produced mainly include MnO₂, Na₃Mn(PO₃)CO₃, and Mn₃O₄. According to the results of high-throughput sequencing, the abundance of Bacillus(0.75%), Acinetobacter(1.26%), Pseudomonas(1.36%), Sphingobacterium(1.81%), Flavobacterium(2.39%), Exiguobacterium(2.97%), Areomonas(7.35%) increases significantly, and the total relative abundance of typical manganese-oxidizing bacterium reaches 17.89%. When the biogenic manganese oxides dosage is 20 mg/L at pH 4.0, EE2 has the best removal efficiency. After 48 h, the removal rate of EE2 reaches 97.7%, in which 76.9% is removed by biogenic manganese oxides.

X522

国家自然科学基金（51878093）