3株高效除磷细菌的分离鉴定及磷去除途径分析
作者:
作者单位:

1.西安建筑科技大学 环境与市政工程学院,西安 710055;2.西安交通大学 人居环境与建筑工程学院,西安 710049

作者简介:

王雨(1997- ),女,主要从事污水微生物处理研究,E-mail:876510091@qq.com。
WANG Yu (1997- ), main research interest: sewage microbial treatment, E-mail: 876510091@qq.com.

通讯作者:

金鹏康(通信作者),男,教授,博士生导师,E-mail:pkjin@hotmail.com。

中图分类号:

X703.1;X172

基金项目:

国家自然科学基金(52070151、51708443);国家重点研发计划(2019YFB2103000);陕西省重点研发计划(2021ZDLSF05-06、2019ZDLNY01-08);陕西省科技创新引领计划(2020CGXNG-021)


Isolation and identification of three high efficiency polyphosphate-accumulating organism and analysis of phosphorus removal routines
Author:
Affiliation:

1.School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, P. R. China;2.School of Human Settlements and Civil Engineering, Xi’an Jiaotong University, Xi’an 710049, P. R. China

Fund Project:

National Natural Science Foundation of China (No. 52070151, 51708443); National Key R&D Program (No. 2019YFB2103000); Shanxi Provincial Key R&D Program (No. 2021ZDLSF05-06, 2019ZDLNY01-08); Shanxi Provincial Science and Technology Innovation Leading Program (No. 2020CGXNG-021)

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    摘要:

    从稳定运行的厌氧/好氧/缺氧序批式反应器(A/O/A-SBR)中筛选分离得到3株高效除磷细菌,通过形态观察、生理生化试验及16S rRNA基因序列分析进行物种分析,确定3株细菌分别为不动杆菌属(Acinetobacter)、克雷伯菌属(Klebsiella)和肠杆菌属(Enterobacter),NCBI保存登录号分别为OL519151、OL519152、OL519153。以pH值、温度及碳源种类作为影响因子,分析细菌生长特性和除磷率,同时分析无机磷和有机磷在细菌胞内、溶解性微生物产物(SMP)及胞外聚合物(EPS)中的分布转化规律。结果表明:在最佳环境条件下,Acinetobacter sp. PK01、Klebsiella sp. PK02和Enterobacter sp. PK03菌株的最高除磷率分别达到89.4%、85.43%和76.95%。其中,Acinetobacter对环境中磷的去除主要依靠吸收胞外无机磷,并以多聚磷酸盐的形式储存于体内,该途径去除了基质中54.93%的磷;Klebsiella对环境中磷的去除主要依靠EPS合成和吸附作用,该途径去除了基质中47.18%的磷;Enterobacter主要依靠多聚磷酸盐和EPS的合成作用,去除了基质中48.32%的磷。

    Abstract:

    In the present study, three strains of high-efficiency phosphorus removal bacteria were screened and isolated from a stable anaerobic/aerobic/anoxic sequencing batch reactor (A/O/A-SBR). The species were identified by morphological observation, physiological and biochemical tests and 16S rRNA sequence analysis. The three strains of bacteria were identified as Acinetobacter, Klebsiella and Enterobacter.The NCBI preservation accession numbers were OL519151, OL519152 and OL519153, respectively. The effects of pH value, temperature and the carbon source on cell grow and phosphorus removal were explored. Meanwhile, the distribution and transformation of inorganic phosphorus and organic phosphorus in bacterial cells, soluble microbial products (SMP) and extracellular polymers (EPS) were also investigated. The results show that the highest phosphorus removal efficiency of Acinetobacter sp. PK01,Klebsiella sp. PK02 and Enterobacter sp. PK03 were 89.4%, 85.43% and 76.95%, respectively, based on the optimal condition. The removal of phosphorus from the environment by Acinetobacter mainly relied on the absorption of extracellular inorganic phosphorus and stored in the body as polyphosphate, removing 54.93% of phosphorus from the matrix. The removal of phosphorus by Klebsiella mainly relied on EPS synthesis and adsorption. This route removed 47.18% of phosphorus from the matrix. Enterobacter removed 48.32% of phosphorus, which mainly depend on the synthesis of polyphosphate and EPS.

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引用本文

王雨,金鹏康,任童,石烜,金鑫.3株高效除磷细菌的分离鉴定及磷去除途径分析[J].土木与环境工程学报(中英文),2023,45(3):196-204. WANG Yu, JIN Pengkang, REN Tong, SHI Xuan, JIN Xin. Isolation and identification of three high efficiency polyphosphate-accumulating organism and analysis of phosphorus removal routines[J]. JOURNAL OF CIVIL AND ENVIRONMENTAL ENGINEERING,2023,45(3):196-204.10.11835/j. issn.2096-6717.2022.063

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  • 收稿日期:2022-02-25
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  • 在线发布日期: 2023-04-29
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