Filtration is a prevalent treatment modality in the domain of wastewater management. Depending on the materials and properties of the filtration media, filtration can be classified into four main categories: microfiltration, ultrafiltration, nanofiltration, and reverse osmosis. The present study focuses on the preparation of a novel porous CaCO? microfiltration membrane, which is based on the microbial-induced calcium carbonate precipitation (MICP) biomineralization process. Initially, CaCO₃ crystal particles with urease activity are prepared by controlling the MICP mineralization process. Secondary microbial mineralization is used to cement the loose calcium carbonate particles, forming a continuous porous solid CaCO? membrane with certain mechanical strength. Filtration tests on bacterial cells, extracellular proteins, and polysaccharides show that the MICP-driven porous CaCO₃ membrane effectively removes Escherichia coli, Brachybacterium sp.， and activated sludge, with removal rates of 99.998%, 99.983%, and 99.996%, respectively. Compared to conventional filter paper, this porous CaCO₃ membrane demonstrates superior capability in removing extracellular polymers (EPS). Furthermore, the CaCO₃ microfiltration membrane prepared using the MICP process also exhibits ideal pore space, non-blocking characteristics, and high permeability.