The nano-zinc titanate (ZnTiO3) photocatalyst was prepared by hydrothermal-assisted sol-gel method. Rhodamine B was used as the target degradant. The effect of initial concentration of Rhodamine B (RhB) on the degradation was analyzed by kinetic model. ZnTiO3 was characterized by SEM, XRD, XRS and UV-Vis DRS. The degradation mechanism was analyzed by free radical trapping experiments. The results show that ZnTiO3 is a pure hexagonal phase with a spherical shape and a particle size of about 50 nm. Under the condition of catalyst dosage of 1g.L^-1, initial concentration of RhB of 5mg.L^-1 and pH of 3, the degradation rate of RhB was 93.2% after photocatalytic reaction for 150 min. Its kinetic equation is k = 0.132C0^-1.253. During the catalytic reaction of ZnTiO3 photocatalyst, OH, h⁺, .O2^- all play a role in the reaction process, and the amount of .OH and h⁺ is similar and more than .O2^-, indicating that .OH and h⁺ play a major role in the catalytic reaction.