In order to determine the start-up method and influence conditions of SBR enhanced biological phosphorus removal (EBPR) granular sludge system and provide theoretical basis for the practical application of EBPR. The start-up and phosphorus removal performance of granular EBPR system and the process of sludge granulation were studied in SBR reactor with an working volume of 12L where sodium acetate was used as carbon source and KH2PO4 was used as phosphorus source. The results showed that when the ratio of carbon to phosphorus in influent water was in lower level(C:P=200:15), and there were other heterotrophic bacteria, the phosphorus removal efficiencies were lower. Compared with 25 ℃, 15 ℃ will increase EPS content of sludge, EPS content of sludge at 25℃ was more than that at 15℃. Moreover, the increase of protein (PN) content was more obvious at 15℃, which was beneficial to the sludge granulation , obvious increase of sludge particle size .And low temperature more favorable for the growth of Rhodocyclus which is mainly responsible for biological phosphorus removal in traditional EBPR system. So that Rhodocyclus became the dominant bacteria in the system and improved the phosphorus removal efficiencies.