Abstract:Taking the aerobic sludge from the wastewater treatment plant as inoculation bacteria, the effects of pH and initial Mn2+ concentration on the activation of manganese-oxidizing bacterium were studied. High-throughput sequencing technology was used to analyze the changes of microbial communities before and after stimulation. On this basis, the biogenic manganese oxides produced by manganese-oxidizing bacterium were characterized by X-ray diffrac-tion. Besides, they were used as oxidants to study the effects of pH and biogenic manganese oxides dosage on the removal of EE2 by biogenic manganese oxides.The results show that the best activation effect of manganese oxidizing bacterium is obtained when the initial Mn2+ concentration is 1mM at pH 7.0 and 83.3% Mn2+ is transformed into biogenic manganese oxides after 7 days. The biogenic manganese oxides produced mainly include MnO2, Na3Mn(PO3)CO3, and Mn3O4.After ac-tivation, the abundance of Bacillus(0.75%), Acinetobacter(1.26%), Pseudomonas(1.36%), Sphingobacterium(1.81%), Flavobacterium(2.39%),Exiguobacterium(2.97%),Areomonas(7.35%) increases significantly, and the total relative frequency of typical manganese-oxidizing bacterium reaches 17.89%. When the biogenic manganese oxides dosage is 20mg/L at pH 4.0, EE2 has the best removal efficiency. After 48h, the removal rate of EE2 reaches 97.7%, in which 76.9% is removed by biogenic manganese oxides.