锰氧化菌激活及生物氧化锰去除乙炔基雌二醇
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作者单位:

1.重庆大学环境与生态学院;2.同济大学环境科学与工程学院

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中图分类号:

X522

基金项目:

国家自然科学基金项目(51878093)


Activation of manganese-oxidizing bacterium and removal of 17α-ethinylestradiol by biogenic manganese oxides
Author:
Affiliation:

1.College of Environment and Ecology, Chongqing University;2.College of Environmental Science and Engineering, Tongji University

Fund Project:

The National Natural Science Foundation of China (NO 51878093)

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    摘要:

    利用污水厂好氧污泥进行了锰氧化细菌的的激活实验,探究pH以及初始Mn2+浓度对激活效果的影响,同时采用高通量测序技术分析最优条件下激活前后微生物群落变化。在此基础上,利用X射线衍射(X-ray diffraction,XRD)技术对产生的生物氧化锰进行了表征。最后研究了pH和投加量对生物氧化锰去除乙炔基雌二醇(17α-ethinylestradiol,EE2)的影响。结果表明,pH=7,初始Mn2+浓度为1mM时,Mn2+氧化率7天内能达到83.3%,锰氧化菌激活效果最好。XRD结果表明,生物氧化锰主要含有MnO2、Mn3O4、Na3Mn(PO3)CO3三种成分。高通量测序结果表明,经过激活后芽孢杆菌属(0.75%)、不动杆菌属(1.26%)、假单胞菌属(1.36%)、鞘氨醇杆菌属(1.81%)、黄杆菌属(2.39%)、微杆菌属(2.97%)、气单胞菌属(7.35%)的丰度显著增加,典型的锰氧化细菌菌属总丰度达到17.89%。EE2在反应pH为4.0,生物氧化锰投加量为20mg/L的条件下去除效果最好,48h后EE2的去除率可达97.7%,其中生物氧化锰对EE2的去除率可达76.9%。

    Abstract:

    Taking the aerobic sludge from the wastewater treatment plant as inoculation bacteria, the effects of pH and initial Mn2+ concentration on the activation of manganese-oxidizing bacterium were studied. High-throughput sequencing technology was used to analyze the changes of microbial communities before and after stimulation. On this basis, the biogenic manganese oxides produced by manganese-oxidizing bacterium were characterized by X-ray diffrac-tion. Besides, they were used as oxidants to study the effects of pH and biogenic manganese oxides dosage on the removal of EE2 by biogenic manganese oxides.The results show that the best activation effect of manganese oxidizing bacterium is obtained when the initial Mn2+ concentration is 1mM at pH 7.0 and 83.3% Mn2+ is transformed into biogenic manganese oxides after 7 days. The biogenic manganese oxides produced mainly include MnO2, Na3Mn(PO3)CO3, and Mn3O4.After ac-tivation, the abundance of Bacillus(0.75%), Acinetobacter(1.26%), Pseudomonas(1.36%), Sphingobacterium(1.81%), Flavobacterium(2.39%),Exiguobacterium(2.97%),Areomonas(7.35%) increases significantly, and the total relative frequency of typical manganese-oxidizing bacterium reaches 17.89%. When the biogenic manganese oxides dosage is 20mg/L at pH 4.0, EE2 has the best removal efficiency. After 48h, the removal rate of EE2 reaches 97.7%, in which 76.9% is removed by biogenic manganese oxides.

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  • 收稿日期:2020-09-26
  • 最后修改日期:2020-11-10
  • 录用日期:2020-11-16
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