巴氏芽孢杆菌生物修复重金属污染溶液试验研究
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华中科技大学 岩土与地下工程研究所

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TU432

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国家重点研发计划 (2018YFC1802302)


Experimental investigation on bioremediation of heavy metal contaminated solution by sporosarcina pasteurii
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Institute of Geotechnical and Underground Engineering,Huazhong University of Science and Technology

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    摘要:

    垃圾填埋场中重金属污染问题严峻,亟待高效的修复技术予以处理。近年来,MICP技术修复重金属的潜力被广泛探究。在众多土著微生物中,巴氏芽孢杆菌脲酶活性和重金属耐受性更佳。但少有研究探究巴氏芽孢杆菌对高浓度重金属的修复潜力,且仅利用MICP理论难以解释巴氏芽孢杆菌对不同重金属的修复机理,此外,常见生物修复工艺的效率有待进一步提高。为避免填埋场中腐殖土和渗滤液物化特性的影响,本研究以重金属污染溶液为研究对象,采用简单拌合法开展生物修复试验;并基于MICP和化学平衡理论,利用Visual MINTEQ软件对修复过程进行简化模拟。结果显示,高浓度范围内,巴氏芽孢杆菌可通过生物矿化和生物富集作用修复Cd、Cr(Ⅲ)和Zn等重金属;此外,简单拌合法操作简便,修复效率高,具有工程应用的潜力。

    Abstract:

    It is urgent to deal with the severe problem of heavy metal pollution in landfills using efficient remediation technology. In recent years, the potential of MICP technology to remedy heavy metals has been extensively explored. Sporosarcina pasteurii shows better urease activity and heavy metal tolerance among the indigenous microorganisms. However, few researches have explored the remediation potential of S. pasteurii to heavy metals in high concentration. The efficiency of commonly used bioremediation processes needs to be further improved. In addition, it is difficult to explain the bioremediation mechanism of S. pasteurii to different heavy metals using only MICP theory. To avoid the impact of humus and leachate in the landfill, this study took heavy metal contaminated solutions as the research object and used simple mixing methods to carry out bioremediation experiments. Based on the MICP and chemical equilibrium theory, the Visual MINTEQ software was used to simplify and simulate the bioremediation process. The results show that in the high concentration range, S. pasteurii can efficiently remedy heavy metal Cd, Cr(Ⅲ) and Zn through biomineralization and bioaccumulation. In addition, the simple mixing method is easy to operate, the bioremediation efficiency is high, and it has the potential for engineering applications.

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  • 收稿日期:2021-06-03
  • 最后修改日期:2021-07-23
  • 录用日期:2021-07-31
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