土豆脲酶的提取及基于EICP技术对风积沙的改良
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作者单位:

1.内蒙古工业大学 土木工程学院;2.内蒙古工业大学土木工程学院;3.内蒙古自治区水利水电勘测设计院;4.内蒙古公路工程咨询监理有限责任公司

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中图分类号:

TU441? ????? ???

基金项目:

国家自然科学基金(51968057);内蒙自然科学基金项目(2019MS05075) ;内蒙古自治区科技计划关键技术攻关项目(2021GG0344)。


Extraction of potato urease and improvement of aeolian sand based on EICP Technology
Author:
Affiliation:

1.School of civil engineering, Inner Mongolia University of Technology;2.Inner Mongolia water resources and Hydropower Survey and Design Institute;3.Inner Mongolia Highway Engineering Consulting and Supervision Co., Ltd.

Fund Project:

National Natural Science Foundation of China (51968057); Inner Mongolia natural science foundation project (2019 MS 05075); Key technology research project of science and technology plan of Inner Mongolia Autonomous Region (2021 GG 0344).

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    摘要:

    基于酶诱导碳酸钙沉淀(EICP)技术使用植物脲酶对沙漠风积沙进行改良加固。为寻求价格低廉和操作简单的脲酶提取方法,实验以土豆为原材料,采用去离子水、甘油水溶液和乙醇水溶液作为提取液,通过破碎冷藏及高速离心的方法将土豆中的植物脲酶进行分离提取,以研究不同提取液对于脲酶提取效果的影响;并利用土豆脲酶液基于EICP技术对风积沙做改良处理,研究结果显示,3种提取液种效果最优的是乙醇溶液,当固液比为1:6和乙醇溶液浓度为30%时,脲酶活性值最高且提取效果最佳。在风积沙改良实验中,试件表面形成了坚硬的结皮层并具有较高的抗压强度,且随着浸泡脲酶次数的增加,无侧限抗压强度也逐渐增强。此研究成果对于沙漠地区的防风固沙工程具有较好的参考意义。

    Abstract:

    Based on enzyme induced calcium carbonate precipitation (EICP) technology, plant urease was used to improve and strengthen desert aeolian sand. In order to find a cheap and simple urease extraction method, potato was used as raw material, deionized water, glycerol aqueous solution and ethanol aqueous solution were used as extraction solution, and the plant urease in potato was separated and extracted by crushing, refrigeration and high-speed centrifugation, so as to study the influence of different extraction solutions on the extraction effect of urease; The results showed that the best effect of the three extracts was ethanol solution. When the solid-liquid ratio was 1:6 and the concentration of ethanol solution was 30%, the urease activity value was the highest and the extraction effect was the best. In the improvement experiment of aeolian sand, a hard crust is formed on the surface of the specimen and has high compressive strength, and the unconfined compressive strength increases gradually with the increase of the number of times of soaking urease. The research results have good reference significance for wind prevention and sand fixation projects in desert areas.

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  • 收稿日期:2021-08-10
  • 最后修改日期:2022-04-27
  • 录用日期:2022-05-19
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