三株高效除磷细菌的分离鉴定及磷去除途径分析
DOI:
作者:
作者单位:

1.西安建筑科技大学;2.西安交通大学

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金 (52070151,51708443); 国家重点研发计划项目 (2019YFB2103000); 陕西省重点研发计划 (2021ZDLSF05-06,2019ZDLNY01-08); 陕西省科技创新引领计划 (2020CGXNG-021)


Isolation and identification of three high efficiency phosphorus removal bacteria and analysis of phosphorus removal characteristics
Author:
Affiliation:

Xi`an University of Architecture and Technology

Fund Project:

National Natural Science Foundation of China (No. 52070151,51708443); National Key R&D Program (No. 2019YFB2103000);Shanxi Provincial Key R&D Program (No. 2021ZDLSF05-06,2019ZDLNY01-08); Shanxi Provincial Science and Technology Innovation Leading Program (No. 2020CGXNG-021)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    本研究从稳定运行的厌氧/好氧/缺氧序批式反应器(A/O/A-SBR)中筛选分离得到了3株高效除磷细菌,通过形态观察、生理生化试验以及16S rRNA基因序列分析进行物种分析,确定3株细菌分别为不动杆菌属(Acinetobacter)、克雷伯菌属(Klebsiella)和肠杆菌属(Enterobacter),NCBI保存登录号分别为OL519151、OL519152、OL519153。以pH值、温度以及碳源种类作为影响因子,分析细菌生长和磷去除效率,同时分析无机磷和有机磷在细菌胞内、溶解性微生物产物(SMP)以及胞外聚合物(EPS)中的分布转化规律。结果表明:在最佳环境条件下Acinetobacter sp. PK01、Klebsiella sp. PK02和Enterobacter sp. PK03菌株的最高除磷效率分别达到了89.4%、85.43%和76.95%。其中,Acinetobacter对环境中的磷去除主要依靠吸收胞外无机磷,并以多聚磷酸盐的形式储存于体内,该途径去除了基质中54.93%的磷;Klebsiella对环境中的磷去除主要依靠EPS合成和吸附作用,该途径去除了基质中47.18%的磷;Enterobacter主要依靠多聚磷酸盐和EPS的合成作用,去除了基质中48.32%的磷。

    Abstract:

    In the present study, three strains of high-efficiency phosphorus removal bacteria were screened and isolated from a stable anaerobic/aerobic/anoxic sequencing batch reactor (A/O/A-SBR). The species were identified by morphological observation, physiological and biochemical tests and 16S rRNA sequence analysis. The three strains of bacteria were identified as Acinetobacter, Klebsiella and Enterobacter.The NCBI preservation accession numbers are OL519151, OL519152, and OL519153, respectively. The effects of pH, temperature and the carbon source on cell grow and phosphorus removal were explored. Meanwhile, the distribution and transformation of inorganic phosphorus and organic phosphorus in bacterial cells, soluble microbial products (SMP) and extracellular polymers (EPS) were also investigated. According to the results, the highest phosphorus removal efficiencies of Acinetobacter sp. PK01,Klebsiella sp. PK02 and Enterobacter sp. PK03 were 89.4%, 85.43% and 76.95%, respectively, based on the optimal condition. The removal of phosphorus from the environment by Acinetobacter mainly relied on the absorption of extracellular inorganic phosphorus and stored in the body as polyphosphate, removing 54.93% of the phosphorus fromthe matrix. The removal of phosphorus by Klebsiella mainly relied on EPS synthesis and adsorption. This route removed 47.18% of the phosphorus from the matrix. Enterobacter removed 48.32% of the phosphorus, which was mainly depended on the synthesis of polyphosphate and EPS.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2022-02-25
  • 最后修改日期:2022-04-27
  • 录用日期:2022-05-23
  • 在线发布日期:
  • 出版日期: