In order to study the flexural performance of the precast steel-concrete stair treads and the comfort of the stairs, four-point compression bending tests on six stair tread specimens were conducted to study the effects of different forms of shear members and the connection methods between the treads and the stair beams on the load-bearing performance of the stair treads. The test results show that, compared with the angle steel and peg shear members, the shear member of channel steel has a greater improvement on the bearing capacity of stair treads; the bearing capacity of welded specimens is little affected by the shear member and is higher than the bearing capacity of simply supported specimens, and the welding can be used as the main way to connect the steel-concrete stair treads to the ladder beam in future engineering applications. ABAQUS software is used to model the stair treads, and the finite element model can accurately simulate the mechanical behavior of the stair treads, achieving the interoperability of the test and simulation. Based on the verification of the model accuracy, the comfort analysis of the overall staircase was carried out. The self-oscillation frequency of the stairs and the acceleration response under human-induced excitation are within the specification limits, which meet the comfort requirements.