As one of the key problems to be solved in the contemporary environmental field, heavy metal pollution, biomineralization technology has become a research hotspot by virtue of its unique potential for heavy metal remediation. Although microorganisms have been studied in heavy metal remediation more deeply, the exploration for plant enzymes, especially soybean urease, in heavy metal management is still insufficient. In this paper, based on the enzyme-induced calcium carbonate precipitation (EICP) technology of soybean urease, heavy metal tolerance test, contaminated solution remediation test and lead and zinc tailings curing test were conducted, and combined with the analysis of ICP, XRD and SEM-EDS test, to study the mineralization effect and mechanism of soybean urease in the treatment of lead-zinc pollution. The study showed that soybean urease showed good tolerance at low concentrations of Pb2+ and Zn2+, and soybean urease was able to adsorb and precipitate heavy metal ions in the solution by inducing the generation of calcium carbonate precipitation and protein complexation, realizing the efficient immobilization of heavy metals; meanwhile, soybean urease wrapped and filled up the tailings by inducing the generation of calcium carbonate, which effectively reduced the leaching of heavy metals, and improved the strength of the tailings.