In the experiment, the denitrifying phosphorus removing bacteria (DPB) was cultivated by high phosphorus and low carbon source influent. It was cultivated successfully by the 2 stage mode for about 100 d. The first stage was about 40 d, which was with the mode of anaerobic/aerobic to enrich the phosphorus accumulating organisms (PAOs). The maximum amounts of the phosphorus release, phosphorus uptake and phosphorus removal were 77.2 mg/L, 89.4 mg/L and 250 mg/L respectively. The second stage was 60 d, which was with the mode of anaerobic/anoxic/aerobic. In this stage, the ratio of denitrifying phosphorus uptake amount to the total phosphorus uptake amount had an ascending tendency. The cultivation of nitrifying activated sludge was about 100 d, with the 50 mg/L ammonia removal amount and more than 98.5% ammonia removal efficiency. Nitrification rate fitted zero order kinetic equation, and the specific nitrification rate constant was 0.002 4 h-1. Phosphorus uptake rates fitted first order absorption kinetic equation. The uptake rate constants under aerobic and anoxic conditions were 0.377 and 0.740 L/(g·h-1). The cultivated DPB sludge and nitrifying activated sludge were used in an anaerobic/anoxic/nitrification sequencing batch reactor（A 2N-SBR） process to remove nitrogen and phosphorus. When the concentrations of influent COD, ammonia and phosphorus were 188.0 mg/L, 54.8 mg/L and 725 mg/L, the removal efficiencies of COD, ammonia and phosphorus were 935%, 76.7% and 94.1% respectively.