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不同强化类型的好氧颗粒污泥结构特性
梁梓轩, 涂倩倩, 苏晓轩, 杨祥宇, 陈俊宇, 陈一, 李宏, 刘彩虹, 何强
重庆大学 三峡库区生态环境教育部重点实验室, 重庆 400044
摘要:
把聚合氯化铝(PAC)、微生物絮凝剂(Microbial flocculant,MBF)、颗粒活性炭(GAC)投加到反应器中可以加快好氧颗粒污泥形成和提升其结构稳定性。扫描电镜(SEM)结果显示,对照组和MBF组好氧颗粒污泥外部呈网状疏松结构,而PAC组和GAC组颗粒表面结构致密。胞外聚合物(EPS)荧光原位染色表明,EPS主要结构组分中的蛋白和β多糖在对照组和MBF组中均为均匀分布,这两组颗粒形成机理符合“EPS假说”;PAC强化型颗粒形成了“蛋白外壳β多糖内核双层构造”,GAC组的颗粒内部有高密度蛋白包裹着颗粒活性炭,这两组颗粒污泥的形成机理更符合“晶核假说”。结构完整性实验表明:PAC、MBF、GAC都可以显著提升好氧颗粒污泥抗水力剪切能力;抗水解酶强弱顺序依次为PAC组 > GAC组 > MBF组 > 对照组。
关键词:  好氧颗粒污泥  聚合氯化铝  微生物絮凝剂  颗粒活性炭  结构完整性
DOI:10.11835/j.issn.2096-6717.2019.119
分类号:X730.1
基金项目:国家自然科学基金(51779020);国家重大科技专项(2015ZX07319-001)
Structural characteristics of different enhanced aerobic granules
Liang Zixuan, Tu Qianqian, Su Xiaoxuan, Yang Xiangyu, Chen Junyu, Chen Yi, Li Hong, Liu Caihong, He Qiang
Key Laboratory of Eco-Environment of Three Gorges Region, Ministry of Education, Chongqing University, Chongqing 400044, P. R. China
Abstract:
Polyaluminum chloride (PAC), microbial flocculant (MBF) and granular activated carbon (GAC) could accelerate the formation of aerobic granular sludge and enhance structural stability. The result of scanning electron microscopy(SEM) showed that the external structure of aerobic granular sludge in control group and MBF group was reticulate and loose, while the PAC group and the GAC group had denser surface structure. And fluorescence in situ staining of extracellular polymers (EPS) showed that the distribution of protein and β-polysaccharide in the main structural components of EPS were the same between the control group and MBF group, which were evenly distributed. The formation mechanism of these two groups consisted to the "EPS hypothesis". PAC-enhanced particles formed a "double-layer structure of protein shell-beta-polysaccharide core". In GAC group, high-density proteins encapsulated granular activated carbon. The formation mechanism of these two groups was more in line with the "crystal nucleus hypothesis". Besides, PAC, MBF and GAC all significantly improved the hydraulic shear resistance of aerobic granular sludge. The capacity of anti-hydrolase resistance was PAC group > GAC group > MBF group > control group.
Key words:  aerobic granular sludge  polyaluminium chloride  microbial flocculant  granular activated carbon  structural integrity
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