In this paper, the Artificial Neural Network (ANN) is applied to the internal force analysis of structure. After introducing the model and algorithm of back-propagation network, a trained BP network of three layers is used to calculate the maximum of elastic moment of middle span of two-way slab. In order to enhance the generalized capability of network, the modified value of weight should be regarded as convergence standard and in order to accelerate the learning process without vibration, the method of adding momentum coefficient is adopted. The analysis program of BP network is carried out with the software of Matlab. The result demonstrates that application of ANN in structure analysis is feasible.