A great quantity of heavy metal cause trouble when the sewage is used in agriculture as fertilizer. An experiment is carried out on green vitriol and sulfur as culture medium, different dosing ratio of the sewage sludge and inoculated or not the sewage sludge, the effect of heavy metal removal by microbe has been studied. It is shown that the microbes nursed under different environment have better effect on the heavy metal removal and the result of experiments is discussed.