In recent years, the problem of fouling on UF membrane mainly centers on the aspect of organic fouling. The cleaning methods of jam caused by fouling of polysulfone UF membrane, the behavior of organic fouling and the elimination of contamination are studied using several chemicals. This experiment proves that NaClO, H2O2 and NaOH all have some effect on the removing organic fouling of UF membrane, of which the H2O2 has the best effect. Controlling the pH value of raw water can decrease the organic fouling of UF membrane. At the same time, a very good result can be gained in the elimination of organic fouling in a way of combining the water solution of NaOH/NaClO and NaOH/H2O2, which makes the infiltration water flux of UF membrane being restored to its initial value. Moreover, the mechanism of organic fouling formation of UF membrane is analyzed.