Eco-environmental water demand in Taohuajiang Valley of Guilin is defined as the minimum water volume required by the valley to maintain the eco-environmental quality not to be degraded over time.By using the site survey data,the eco-environmental water demand in Taohuajiang valley is studied preliminarily.The results calculated indicate that: the out-stream eco-environmental water demand is about 14.9 million m3 per year,about 4 percent of annual runoff of the valley;and the in-stream eco-environmental water requirement is about 109.8 million m3 per year,about 30 percent of annual runoff.This study provides scientific basis for local sustainable water resources use and management as well as for eco-environmental water requirement study in similar regions in terms of Karst geological condition and climate.