In recent years wood trusses are widely used in buildings due to their excellent performance and ease of construction. As with solid lumber joists, however, the vibration of wood truss joist floors can cause occupants to feel discomfort. To understand the relationship between the vibration performance of wood truss joist floors and the perceptions of occupants, experimental and subjective evaluations were conducted on six truss joist floors. The results show there is close relationship between perceptions of occupants and the fundamental natural frequency, static deflection and vibration acceleration of truss joist floors. The installation of bracings and straps greatly improved the vibration performance of light truss joist floors. We suggest that two or more factors should be included in the design criteria for controlling wood floor vibration, such as static deflection and fundamental natural frequency. Peak acceleration can be adopted as a factor in the evaluation system of vibration performance of wood truss joist floors.