A threestage seriesoperational pilot scale constructed wetland was built for domestic wastewater treatment with inflow of 4.5 m3/d .The mass balance method was adopted to quantify the transportation and transformation of pollutants in constructed wetland during steadystate operation.It was shown that the average removal rate of CODcr was 87.14%, in which 68.45% contributed by dissimilation and 18.69% contributed by assimilation.The average removal rate of TN was 60.21% and the contributions of denitrification and microbial assimilation or plant assimilation were 53.64%, 5.39% and 1.18%, respectively.The average removal rate of TP was 59.36% and the contributions of substrates absorption and microbial assimilation or plant assimilation were 43.55%, 12.26% and 3.55%,respectively.In conclusion, microorganisms played a key role in the removal of pollutants and substrates adsorption was the main approach to phosphorus removal.