A strain that have capable of metabolizing phenol as a sole source of carbon and energy sources was isolated from an sludge collected from textile chemical plant in Taiwan. It was identified as Pseudomonas sp. based on its morphology, physiological, biochemical characteristics with an automatic biometrical system and the 16S rDNA sequence analysis. It is shown that the biodegradation of phenol was optimized at culture medium pH 8.5 and 30℃.Under the condition, the strain degraded phenol completely within 17h even though the initial phenol level was high to 500 mg/L. With the study of dynamic growth kinetics of suspended cells, the value of μmax, KS and KI were found to be 0.452 h-1, 28.617 mg/L, 782.4 mg/L for strain CN 6 degrading phenol using Haldane's inhibition kinetic models.