To study their resistance performance, totally 222 specimens of panel-to-lumber nailed joints, in different configurations and used in light wood frame construction, were manufactured and tested under monotonic load. There are five kinds of destruction produced with nailed joints,which are model M-c1, rupture of panel, shear failure of panel, wood tear-off and push-through of nail. The resistance of nailed joints with vertical nailing is higher than that with toe-nailing, while the stiffness is almost the same. The angle between the panel orientation and dimension lumber wood grain affects the resistance and the stiffness of nailed joints little, while the angle between loading and dimension lumber wood grain affects the stiffness but not the resistance. The empirical equations to calculate the stiffness of the nailed joints are derived, thus providing reference for evaluating the performance of shear walls and diaphragm (floor and roof) in design of light wood frame structures.