Urban surface pollutant is now recognized as a major source of urban non-point pollution, thus, it is a key problem to scientifically understand and compute surface pollutants. In order to obtain the build–up law, starting in May, 2006, a study was conducted to measure surface pollutants from representative land uses types in Zhenjiang, Jiangsu Province. It is shown that surface pollutants increase to the maximum value gradually and change with the periodic variation. Based on the storage-input-output balance principle, the input speed coefficient and removal rate coefficient are introduced to establish the build -up model of surface pollutants. By fitting with the measured data and comparing with other models, the precision of the model is accurate, and the calculated results can be used as the input of the wash-off model, which provides the scientific basis for non-point source pollution control and management.