Taking a two-lane extra large bridge as sample,it is to analyze the real time thermal field and thermal effect on a long-span prestressed concrete box girder. The obtained data is compared with the simulation which is based on a balancing theory of temperature distributions and exchange in box girder exterior surfaces. The results show that the proposed method can emulate the actual boundary conditions precisely, it can satisfy the design analysis requirement of the practice engineering. Based on the emulation of the temperature field, with the ANSYS secondary development technology, FEM modules are developed to analyze the thermal field of the prestressed concrete box girder bridges. The present examples show that the developed modules are effective and useful.