Seepage resistance and moisture proof of concrete structure is a hard work in civil engineering. The influence factors of seepage resistance and moisture proof of concrete using ElectroOsmotic Pulse (EOP), such as electric voltage, water to cement ratio of concrete, ion concentration in liquid phase, and pulse frequency, were analyzed. Simulating experiment of EOP used to drain water and reduce relatively humidity of concrete structure is executed. The results show that, volume of water seepage is improved due to application of EOP in concrete with increase of electric voltage, W/C of concrete, and concentration of sodium sulfate. And pulse frequency has little effect on volume of water seepage. The results are also presented that effect of dewatering and humidity reducing are remarkable when EOP is applied in concrete structure.