A novel three-dimensional isolation bearing (3DIB) was proposed, based on respective mechanical characteristic of lead rubber bearing, combined disc springs and rhombic steel plated damper. Firstly, the configuration department and mechanical design methodology was introduced; the horizontal and vertical stiffness and energy dissipation performance test was performed on a full scale three-dimensional isolation bearing, respectively. The results indicated that the proposed three-dimensional isolation bearing has reasonable configuration and explicit mechanical transmission mechanism; appropriate stiffness and energy dissipation performance can be achieved by reasonable design, keeping favorable holistic stability at plentiful horizontal shear displacement.