To deal with failure of wet joint between a closure segment and its neighboring segments of main girder in an existing prestressed concrete cable-stayed bridge, the technology on replacement of closure segment was presented. In order to analyze structural characteristics of this technology, its technical approaches and construction key points were introduced. Then a FEM model for a typical prestressed concrete cable-stayed bridge was established, and a construction stage analysis method was given in detail, including establishing a structural basement calculation model for replacement of closure segment and simulating its construction procedures. Based upon calculation results, variations of some structural state parameters with construction stages, such as deck deflection of main girder, longitudinal horizontal displacement at the top of pylons, cable forces, were analyzed. The results showed, this method could reflect actual change process of structural state and existing inspected results of cable-stayed bridge, and FEM results could well meet with construction monitoring results during replacement of closure segment. Thus variations of structural characteristics with construction stages could actually be revealed. This method could provide references for design and construction control of replacement of closure segment in existing prestressed concrete cable-stayed bridges.