In order to investigate the influence factor of short cut nitrification and denitrification, the rapid startup and steady operation of the short cut nitrification and denitrification with real time control were studied. The results show that the short cut nitrification and denitrification can be rapidly setup by the control of DO and pH cooperated with low DO. The excessive aeration has negative effect on the accumulation of NO -2-N for primary short cut nitrification and denitrification sludge. With real time control and reasonable aeration time, the ammonia can be oxygenated completely and the nitrite can not be oxygenated when aeration stops and ammonia oxygenation just completes. Thus the real time control strategy not only realizes the short cut nitrification and denitrification, but also maintains such steady operation.