The dynamic finite element model (FEM) of a prestressed concrete continuous girder bridge (PCCGB), named Zhangjiagang river main bridge, was established and updated based on the results of field ambient modal testing using real-coded accelerating genetic algorithm (RAGA), which objective function was defined based on frequency index and correlation coefficient index for evaluating the updated FEM. The dynamic FEM of the bridge was updated based on seven experimental modal parameters. The prediction ability of the updated FEM were evaluated based on three experimental modal parameters. The updated results and prediction ability of updated FEMs indicated that they can reflect adequately the dynamic characteristics of actual PCCGB by using the above objective function and RAGA.