Based on the modal assurance criterion, using simulated annealing algorithm, the dynamic characteristic data of cable-stayed bridge are extracted, optimal acceleration sensor layout scheme of main beam is obtained. According to this optimization scheme, we carry out a dynamic characteristics test on one cable-stayed bridge. The results between test data and finite element modal calculation are very closely. Theory technique research and field modal test demonstrated that the simulated annealing algorithm based on modal assurance criterion is effective and viable for optimal acceleration sensors placement of cable-stayed bridge.