Ventilation design is the difficulty of transformer room design. Temperature field and velocity field in a transformer room was simulated by CFD method . By comparing simulation value with the experimental value in related document , the effectiveness of the mathematical model was verified. Based on it, an indoor substation transformer built was taken as the simulation object, then designed and simulated six kinds of ventilation condition by changing the location and area of air inlet, focused on the influence of air inlet on the ventilation effect by comparing the temperature field ,velocity field and temperature characteristic value in different condition. The simulation results show that when air inlet area is fixed, the air inlet should be arranged in the radiator side and its center height should be controlled in the radiator center height or slightly offset position, it should not be higher than the radiator. When the air inlet location is fixed, air inlet area should be increased along the high direction to improve ventilation effect. The conclusions can provide technical support for the ventilation design of transformer room.