Five color temperature LEDs were compared to traditional light sources (HPS and MH) under three lighting levels and negative contrasts to study the effect of light sources color on tunnel lighting. Through the experiment of reaction times, the relation between light sources color and reaction times, and the relation between light sources color and pupil size were studied, respectively. The experiment result shows that visual performance and cirtopic with high color temperature LED are the most significant under high lighting level. The visual performance and cirtopic with middle color temperature LED are the most significant under mesopic lighting level. It is shown that comprehensive influence of light sources color on visual performance and cirtopic must be considered for choosing appropriate light sources in different sections of tunnel lighting.