In this paper， the experimental study on the self-tapping screw's pullout resistance in the wood structure was conducted. Domestic ordinary screws were used together with imported or domestic wood to fabricate 6 screw connection specimens in wooden walls. Then monotonic loading tests were conducted and it can be concluded that， the common round screw connection strength mainly depends on the failure mode， the lack of cooperation effect significantly influences the strength of screw connections， and the dispersion of screw connection stiffness is high. So， to obtain formulae for the self-tapping screw connection strength and stiffness by further experimental study will be very necessary.