The bench test of diesel engine is conducted fueled with biodiesel, diesel and blending fuel, the changes of cylinder pressure with crank angle is also measured, combined with the measurement results of carbonyl emissions, the theoretical relationship between combustion process and carbonyl formation is analyzed. The results show that, combustion intermediates such as C7, C5, CO and C2H2 are produced in the fuel oxidation process during the premixed combustion phase. The diffusion combustion period extend with the increase of load, and it is the main stage of re-oxidation and decomposition for the intermediate products, the cylinder temperature and pressure increase with the increase of load, while the ignition delay period is shortened, the probability of oxidation and conversion for carbonyl pollutants improve because of the extended residence time in the high-temperature and high pressure region, the high exhaust temperature in high load is conducive to the re-oxidation of carbonyl emissions.