The temperature distributions were measured in hardening concrete slabs, determining final set time of concrete; the calculation methods of built-in temperature difference and the effective temperature difference were provided to determine the corresponding values of the slabs constructed during spring and fall, respectively. Finally, the influence of built-in temperature difference on slab curling and stress were evaluated based on finite element analysis. The results show that negative built-in temperature differences were found in both spring and fall construction which will lead to loss of slab support in the mid-slab position during the most time of a day, and larger tensile stress generates at the slab bottom. However, small size slab will minimize this negative effect from built-in temperature difference and at the same time reduce fatigue damage.