Under Fire conditions， the interface thermal resistance between steel and concrete has important influence on structure temperature field. TheINSTRON 8874 material testing machine for high temperature thermal contact resistance testing device was used to conduct the thermal contact resistance experiments of steel and concrete interface. According to the time history curve of each measuring point's temperature，the interface temperature near steel and near concrete was obtained using the polynomial fitting and extrapolation method. The interface thermal contact resistance was calculated by heat conduction equation and definition of thermal contact resistance. The test results showed that under different interface pressure，the thermal contact resistance value was constant and comparatively consistent with the literature; Without interface pressure，the thermal contact resistance value was unstable and varied obviously with temperature.