In structure design, Anti-seismic design is an indispensable step. This paper takes China Comic and Animation Museum as an example, First floor adopts steel mesh tube-steel frame-supporting(steel plate shear wall)structure. Second floor and above adopt the steel frame-support (steel plate shear wall) + cross layer truss structure. Existing structure Anti-seismic theory and finite element analysis were used for the structure of the dynamic and static elastoplastic analysis. Analysis shows that the structure is in a good lateral stiffness and has better seismic performance.