To improve the strengthening effect and reliability of concrete structure strengthened with carbon fiber sheets, a new hybrid bonding pre-stressed carbon fiber sheets technique is developed. This technique combines the pre-stressed carbon fiber sheets with mechanical fastening of pre-screwed bolts. In accordance with the fracturing characteristics and the strengthening method of aging RC beam bridges, different carbon fiber sheets strengthening technology were applied on the intact and destructive RC beams. And a series of experiments were conducted to examine and assess the flexural performance of RC beams. Then, a new type of tensioning equipment was invented to meet the need of tensioning carbon fiber sheets in practical engineering. The experiment results show that the hybrid bonding pre-stressed carbon fiber sheets is a more reliable strengthening technique which can not only improve the flexural capacity and the section stiffness of RC beams during normal use stage, but also can increase the cohesive force between carbon fiber sheets and concrete interface. It can make full use of the tensile strength of carbon fiber sheets and can restrain the opening and developing of the cracks.