The effect of knot was analyzed on stability of glulam column under Axial Compressive Loading, using the column deflection curve method (CDC method). The initial bending, eccentricity, wood knots and elastic-plasticity of wood were considered for analysis. The member in the model was divided into three areas:knot area, knot influence area and no influence area, and three related constitutive relations were adopted in the process of analyzing. The locations of knots were randomly generated by designed program to simulate the randomly distributed knots in practical components. The stability coefficient φ was calculated considering knot at the rate of 2%, 6% and 9%, respectively. The results showed that the knots could reduce the stability capacity of the glulam column in axial compression in some degree. The numerical data was further compared with the test data and they were fit well each other.