This paper focuses on the physiological adaption and subjective sensation in cold acceptable environment zone between male college students of southern and northern of China. 16 subjects of southern China and 16 subjects of northern China were selected, to test their physiological parameters including skin temperature, heart rate (HR), heart rate variability (HRV), blood volume pulse (BVP) and electromyogram (EMG), and to ask the subjects to fill in questionnaires. The experimental results revealed that:In terms of all subjective questions, southern subjects give a better response than northern subjects; and have a higher average skin termperature and EMG value and a lower HR、LF/HF values than northern subjects; From neutral environment to cold acceptable environment zone, the experimental period of appearing the significant difference of subjective perception index differential mean and physiological response index differential mean of southern subjects are later or less than northern residents. Conclusions are:1) north subjects are more sensitive to cold environment, which are weaker to cold tolerance, have weak cold acclimation and have strong physiological regulation phenomenon; 2) Southern subjects have better tolerance to environment, which lead to result of small physiological regulation; they have a strong cold acclimation ability and their basic metabolism and non-shivering thermogenesis (NST) are slightly higher than the northern.