To study the pulse effect on the seismic response of steel coal-conveyer gallery in thermal power plant, dynamic analysis of a typical coal-conveyer gallery structure was carried out in this paper. Firstly, the energy-based pulse-like ground motion identification method was adopted to select nine ground motions with different pulse periods. Secondly, the corresponding ground motions without pluses were obtained by using standard mathematical pulse model to remove the pulses from the selected pulse-like ground motions, and then, a comparative analysis was conducted considering the ground motions with and without pulses. Results show that pulse-like ground motions can significantly affect the structural responses, especially when the pulse period is close to the fundamental period of the structure. The pulse effect on the responses of coal-conveyer gallery is most obvious when the three-direction input is used, and the maximum value of amplification factor is 2.1. Under the pulse-like ground motions, the displacements of column top is 1.36 times of the elastoplastic displacement limit, which will cause serious damage beyond expectations. The mid-span deflection of steel truss is 2.97 times of the serviceability limit, which may adversely affect the serviceability of the structure and the safety of the attached equipment. Therefore, in seismic design, to avoid misleading estimates of the responses of structure, it is suggested that the pulse effect and the multi-dimensional input should be considered cautiously for the coal-conveyer gallery in the neat fault region.