The Local Climate Zone (LCZ) scheme aims to provide an objective and standardized classification protocol for urban temperature studies in any city. The field temperature data (Jul.-Sept., 2016) from 12 LCZ sites located in urban and rural areas of Nanjing, China, was used to evaluate the LCZ scheme. For the 12 LCZ sites, the features of heat island and the differences of daily max/min temperature and heating/cooling rate were analyzed. The UHI intensity increases rapidly after sunset, reaching at maximum about three to five hours later, and then declines gradually, which is the result of the variation of the heating/cooling rates of a LCZ site. It was observed that the higher level of urbanization the stronger heat island effect for the LCZ sites. The heat island magnitudes may even be negative during the midday period. It was shown that the water area (LCZ G) produces cooling effect during the daytime as expected while shows warming effects during the night time. The daily maximum temperature difference between urban and rural occurs in the minimum rather than in the maximum. Distinct differences in air temperature among the LCZ sites reflect the thermal effects of local surrounding properties.