This paper aims to study the mechanical performances of reinforced concrete two-way slab with different stud parameters. The experimental study on seven reinforced concrete two-way slab-column connections with punching shear studs was conducted. The main parameters investigated were spacing of stud, the diameter of the stud, the height of the stud, the strength of the stud and the arrangement of the stud. The whole process of damage of slab-column connections with studs was simulated using static loading method. The results show that increasing the diameter of the stud, the height of the stud or the strength of the stud can improve the anti-punching shear performance of the specimen. The increase in diameter is the most obvious, and the decrease in deformation is up to 36%. Increasing stud spacing and reducing stud height weaken the load-bearing capacity and thus the deformation are increased. By comparing three different stud layouts, it is found that using radiation type contributes better to the improvement of the specimen anti-punching shear capacity, and the former was more economical. Then, the accuracy of the test results was verified by comparing the calculated results of relevant specifications with the test results. The results show that the safety reserve of specification calculation results of slab-column structure with studs is sufficient. However, it is not enough to reflect the nature of test results, especially contributions of the stud to anti-punching shear performance of slabs are underestimated.