Vertical vibration would be incurred easily under the pedestrian-elevator load in the cantilevered floor with erecting escalators. The cantilevered floor in a commercial interlayer is used as an example to study the vertical vibration response considering the influence from the escalator operation, the number of pedestrians and the walking direction.Based on the test data, we evaluate the comfort of the cantilevered floor and the perception of personnel. Results show that the pedestrian frequency is the primary factor affecting the vibration of the cantilevered floor, while the operation of escalator is a secondary factor. It is also found that compared with the unidirectional walking, the vibration response of the cantilevered floor becomes to be weakened obviously when people are walking on the escalator in two-way by the same step. The peak acceleration is used to evaluate the comfort of the cantilevered floor. It is revealed that when the pedestrian runs on the escalator, the vibration response of the cantilevered floor is easy to exceed the comfort limit. And we use the KB (Konstant Beurteilungswerte) value to evaluate the comfort of cantilevered floor. When the pedestrian is stationary relative to the escalator, the vibration of the cantilevered floor does not exceed the perceptibility allowable value. However, when the pedestrian walks or moves relative to the escalator, the vibration of the cantilevered floor will exceed the threshold value.