Rapid urbanization and global warming aggravate the thermal discomfort of urban outdoor environment and increase the risk of thermal security. In order to find out the physiological parameters changes of human body in outdoor thermal environment, provide a basis for rapid urban thermal environment evaluation, an outdoor experiment was carried out to observe the thermal response of human body. Based on the measured data, the simulation precision analysis and coincidence test of the two-node model were carried out. It is found that human skin temperature and body core temperature show different trends under the influence of outdoor wind speed, MRT and self-regulation, in order to ensure the simulation accuracy, the two-node model of human body needs to be modified from the following four aspects:skin and core temperature, standard human model, temperature of regulation starting, convective heat transfer coefficient, relevant parameters and calculation process shall be written as assignable variables or mathematical expressions, the revised two-node model is universal and applicable to the prediction of outdoor human thermal response.